5.1 Nutrients level

5.1.1 Nutrient concentration

5.2 Direct effects of nutrient enrichment

5.2.1 Chlorophyll concentration

5.2.2 Water transparency

5.2.3 Abundance of macroalgae

5.2.4 Shift in floristic species composition

5.3 Indirect effects of nutrient enrichment

5.3.1 Abundance of seaweeds and seagrasses

5.3.2 Dissolved oxygen

BLK-RO-RG-Coast: The Reference Condition for the metrics Phytoplankton Numerical abundance [cells/l] , Total Biomass [mg/m3] , chlorophyll a [µg/l], MEC% and DE% are derived from historical data from the relatively reference period for the Black Sea (RO)
BLK-RO-RG-Coast: WFD / Directive 2000/60/EC; For statistical analyses of data the biotic indices can be used (AMBI, M-AMBI). The index AMBI has been applied to different European geographical areas, experiencing various human impact . The AMBI offers a pollution classification of a particular site, representing the benthic community health . For the soft-bottom macrofauna the species could be classified into five ecological groups, according to their sensitivity/tolerance to an increasing stress gradient. For getting a correct evaluation of the ecological status of benthic communities can be used another method of calculation such as M-AMBI index (Multivariate AMBI) In the process of M-AMBI calculation, it takes into account the values of AMBI, richness (number of species) and Shannon’s diversity ( H’).
BLK-RO-RG-Marine: The approach used for determining GES is by providing a qualitative description of what GES will look like when achieved for the Descriptor 8. It focuses on Annex I and takes account of Annex III MSFD and COM Decision 2010/477/EU.
BLK-RO-RG-Marine: The approach used for determining GES is by providing a qualitative description of what GES will look like when achieved for the Descriptor 5. It focuses on Annex I and takes account of Annex III MSFD and COM Decision 2010/477/EU.
BLK-RO-RG-Marine: In determining GES_Macroalgae the EEI index is used. The index is applied in a modified form adapted to the Black Sea conditions (with fewer number of phytobenthic species comparing with other seas), so the results are expressed using the wet biomass of the identified species, and not the number of species like in the original form of the index. Each identified species was included in ecological groups, as follows: ESG I - perennial species indicator of less polluted areas and ESG II - opportunistic species able to develop and thrive in eutrophic areas (eg. Ceramium, Cladophora, Ulva). Wet biomass was calculated for each species and reported to square meter. In order to calculate the EEI index, is necessary to determine the average fresh biomass for the tolerant and opportunistic species. An EEI index is determined for each transect, and the final value is the average between the obtained values. The two perennial species with great ecological importance for the Romanian seaside - Cystoseira barbata and Zostera noltii represent a suitable substrate for the development of a large number of epiphytes, so in order to have a correct estimate of the biomass of these species, epiphytic flora was removed and weighed separately.
BLK-RO-RG-Coast: For coastal waters, good ecological status for the descriptor 8, will be defined by framing concentrations of priority hazardous substances synthetic and non-synthetic in limits of Directive 2008/105/EC. The method used consists in comparing annual averages with the requirements under Directive 2008/105/EC.
BLK-RO-RG-Transitional: For transitional waters, good ecological status for the descriptor 8, will be defined by framing concentrations of priority hazardous substances synthetic and non-synthetic in limits of Directive 2008/105/EC. The method used consists in comparing annual averages with the requirements under Directive 2008/105/EC.
BLK-RO-RG-Coast: For coastal waters good ecological status for descriptor 5, will be defined by framing concentrations of nutrients and oxygen regime in threshold values ​​established in "Studies on the development of classification and comprehensive assessment of the status of surface water (transitional waters and coastal waters) as required by the Water Framework Directive 2000/60/EC on the basis of biological chemical and hydromorphological elements", for natural water bodies and heavily modified water bodies", developed by the National Institute for Marine Research and Development,,Grigore Antipa". The method used consists in comparing annual averages whit values ​​determined in these studies.
BLK-RO-RG-Transitional: For transitional waters good ecological status for descriptor 5, will be defined by framing concentrations of nutrients and oxygen regime in threshold values ​​established in "Studies on the development of classification and comprehensive assessment of the status of surface water (transitional waters and coastal waters) as required by the Water Framework Directive 2000/60/EC on the basis of biological chemical and hydromorphological elements", for natural water bodies and heavily modified water bodies", developed by the National Institute for Marine Research and Development,,Grigore Antipa". The method used consists in comparing annual averages whit values ​​determined in these studies.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined comparing the distributional range in the reference period with the present distributional range.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined by comparing its extent when evaluated against the threshold given for the extent of that habitat.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined by attaining the thresholds set for density / biomass / population structure of the species that are characteristic, key or structural for that particular habitat type.
BLK-RO-RG-Coast: Virtual population analysis for fishing stock assessment (XSA, ICA - SPR, XSA - TUR, VIT4WIND - DGS, VPA XSA - ANE, WHG)
BLK-RO-RG-Marine: Good conservation status for dolphin population will be defined comparing the dolphin stocks with the baseline.
BLK-RO-RG-Coast: Good conservation status for the Cystoseira barbata will be defined comparing the present distributional and population densities range with the baseline.
BLK-RO-RG-Coast: Good conservation status for the Zostera noltii will be defined comparing the present distributional and population densities range with the baseline.
BLK-RO-RG-Coast: For benthic communities (Mytilus galloprovincialis, Modiolula phaseolinus and Lentidium mediterraneum) good environmental status will be defined comparing the present distributional and population densities range with the baseline.

BLK-RO-RG-Coast: The Reference Condition for the metrics Phytoplankton Numerical abundance [cells/l] , Total Biomass [mg/m3] , chlorophyll a [µg/l], MEC% and DE% are derived from historical data from the relatively reference period for the Black Sea (RO)
BLK-RO-RG-Coast: WFD / Directive 2000/60/EC; For statistical analyses of data the biotic indices can be used (AMBI, M-AMBI). The index AMBI has been applied to different European geographical areas, experiencing various human impact . The AMBI offers a pollution classification of a particular site, representing the benthic community health . For the soft-bottom macrofauna the species could be classified into five ecological groups, according to their sensitivity/tolerance to an increasing stress gradient. For getting a correct evaluation of the ecological status of benthic communities can be used another method of calculation such as M-AMBI index (Multivariate AMBI) In the process of M-AMBI calculation, it takes into account the values of AMBI, richness (number of species) and Shannon’s diversity ( H’).
BLK-RO-RG-Marine: The approach used for determining GES is by providing a qualitative description of what GES will look like when achieved for the Descriptor 8. It focuses on Annex I and takes account of Annex III MSFD and COM Decision 2010/477/EU.
BLK-RO-RG-Marine: The approach used for determining GES is by providing a qualitative description of what GES will look like when achieved for the Descriptor 5. It focuses on Annex I and takes account of Annex III MSFD and COM Decision 2010/477/EU.
BLK-RO-RG-Marine: In determining GES_Macroalgae the EEI index is used. The index is applied in a modified form adapted to the Black Sea conditions (with fewer number of phytobenthic species comparing with other seas), so the results are expressed using the wet biomass of the identified species, and not the number of species like in the original form of the index. Each identified species was included in ecological groups, as follows: ESG I - perennial species indicator of less polluted areas and ESG II - opportunistic species able to develop and thrive in eutrophic areas (eg. Ceramium, Cladophora, Ulva). Wet biomass was calculated for each species and reported to square meter. In order to calculate the EEI index, is necessary to determine the average fresh biomass for the tolerant and opportunistic species. An EEI index is determined for each transect, and the final value is the average between the obtained values. The two perennial species with great ecological importance for the Romanian seaside - Cystoseira barbata and Zostera noltii represent a suitable substrate for the development of a large number of epiphytes, so in order to have a correct estimate of the biomass of these species, epiphytic flora was removed and weighed separately.
BLK-RO-RG-Coast: For coastal waters, good ecological status for the descriptor 8, will be defined by framing concentrations of priority hazardous substances synthetic and non-synthetic in limits of Directive 2008/105/EC. The method used consists in comparing annual averages with the requirements under Directive 2008/105/EC.
BLK-RO-RG-Transitional: For transitional waters, good ecological status for the descriptor 8, will be defined by framing concentrations of priority hazardous substances synthetic and non-synthetic in limits of Directive 2008/105/EC. The method used consists in comparing annual averages with the requirements under Directive 2008/105/EC.
BLK-RO-RG-Coast: For coastal waters good ecological status for descriptor 5, will be defined by framing concentrations of nutrients and oxygen regime in threshold values ​​established in "Studies on the development of classification and comprehensive assessment of the status of surface water (transitional waters and coastal waters) as required by the Water Framework Directive 2000/60/EC on the basis of biological chemical and hydromorphological elements", for natural water bodies and heavily modified water bodies", developed by the National Institute for Marine Research and Development,,Grigore Antipa". The method used consists in comparing annual averages whit values ​​determined in these studies.
BLK-RO-RG-Transitional: For transitional waters good ecological status for descriptor 5, will be defined by framing concentrations of nutrients and oxygen regime in threshold values ​​established in "Studies on the development of classification and comprehensive assessment of the status of surface water (transitional waters and coastal waters) as required by the Water Framework Directive 2000/60/EC on the basis of biological chemical and hydromorphological elements", for natural water bodies and heavily modified water bodies", developed by the National Institute for Marine Research and Development,,Grigore Antipa". The method used consists in comparing annual averages whit values ​​determined in these studies.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined comparing the distributional range in the reference period with the present distributional range.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined by comparing its extent when evaluated against the threshold given for the extent of that habitat.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined by attaining the thresholds set for density / biomass / population structure of the species that are characteristic, key or structural for that particular habitat type.
BLK-RO-RG-Coast: Virtual population analysis for fishing stock assessment (XSA, ICA - SPR, XSA - TUR, VIT4WIND - DGS, VPA XSA - ANE, WHG)
BLK-RO-RG-Marine: Good conservation status for dolphin population will be defined comparing the dolphin stocks with the baseline.
BLK-RO-RG-Coast: Good conservation status for the Cystoseira barbata will be defined comparing the present distributional and population densities range with the baseline.
BLK-RO-RG-Coast: Good conservation status for the Zostera noltii will be defined comparing the present distributional and population densities range with the baseline.
BLK-RO-RG-Coast: For benthic communities (Mytilus galloprovincialis, Modiolula phaseolinus and Lentidium mediterraneum) good environmental status will be defined comparing the present distributional and population densities range with the baseline.

BLK-RO-RG-Coast: The Reference Condition for the metrics Phytoplankton Numerical abundance [cells/l] , Total Biomass [mg/m3] , chlorophyll a [µg/l], MEC% and DE% are derived from historical data from the relatively reference period for the Black Sea (RO)
BLK-RO-RG-Coast: WFD / Directive 2000/60/EC; For statistical analyses of data the biotic indices can be used (AMBI, M-AMBI). The index AMBI has been applied to different European geographical areas, experiencing various human impact . The AMBI offers a pollution classification of a particular site, representing the benthic community health . For the soft-bottom macrofauna the species could be classified into five ecological groups, according to their sensitivity/tolerance to an increasing stress gradient. For getting a correct evaluation of the ecological status of benthic communities can be used another method of calculation such as M-AMBI index (Multivariate AMBI) In the process of M-AMBI calculation, it takes into account the values of AMBI, richness (number of species) and Shannon’s diversity ( H’).
BLK-RO-RG-Marine: The approach used for determining GES is by providing a qualitative description of what GES will look like when achieved for the Descriptor 8. It focuses on Annex I and takes account of Annex III MSFD and COM Decision 2010/477/EU.
BLK-RO-RG-Marine: The approach used for determining GES is by providing a qualitative description of what GES will look like when achieved for the Descriptor 5. It focuses on Annex I and takes account of Annex III MSFD and COM Decision 2010/477/EU.
BLK-RO-RG-Marine: In determining GES_Macroalgae the EEI index is used. The index is applied in a modified form adapted to the Black Sea conditions (with fewer number of phytobenthic species comparing with other seas), so the results are expressed using the wet biomass of the identified species, and not the number of species like in the original form of the index. Each identified species was included in ecological groups, as follows: ESG I - perennial species indicator of less polluted areas and ESG II - opportunistic species able to develop and thrive in eutrophic areas (eg. Ceramium, Cladophora, Ulva). Wet biomass was calculated for each species and reported to square meter. In order to calculate the EEI index, is necessary to determine the average fresh biomass for the tolerant and opportunistic species. An EEI index is determined for each transect, and the final value is the average between the obtained values. The two perennial species with great ecological importance for the Romanian seaside - Cystoseira barbata and Zostera noltii represent a suitable substrate for the development of a large number of epiphytes, so in order to have a correct estimate of the biomass of these species, epiphytic flora was removed and weighed separately.
BLK-RO-RG-Coast: For coastal waters, good ecological status for the descriptor 8, will be defined by framing concentrations of priority hazardous substances synthetic and non-synthetic in limits of Directive 2008/105/EC. The method used consists in comparing annual averages with the requirements under Directive 2008/105/EC.
BLK-RO-RG-Transitional: For transitional waters, good ecological status for the descriptor 8, will be defined by framing concentrations of priority hazardous substances synthetic and non-synthetic in limits of Directive 2008/105/EC. The method used consists in comparing annual averages with the requirements under Directive 2008/105/EC.
BLK-RO-RG-Coast: For coastal waters good ecological status for descriptor 5, will be defined by framing concentrations of nutrients and oxygen regime in threshold values ​​established in "Studies on the development of classification and comprehensive assessment of the status of surface water (transitional waters and coastal waters) as required by the Water Framework Directive 2000/60/EC on the basis of biological chemical and hydromorphological elements", for natural water bodies and heavily modified water bodies", developed by the National Institute for Marine Research and Development,,Grigore Antipa". The method used consists in comparing annual averages whit values ​​determined in these studies.
BLK-RO-RG-Transitional: For transitional waters good ecological status for descriptor 5, will be defined by framing concentrations of nutrients and oxygen regime in threshold values ​​established in "Studies on the development of classification and comprehensive assessment of the status of surface water (transitional waters and coastal waters) as required by the Water Framework Directive 2000/60/EC on the basis of biological chemical and hydromorphological elements", for natural water bodies and heavily modified water bodies", developed by the National Institute for Marine Research and Development,,Grigore Antipa". The method used consists in comparing annual averages whit values ​​determined in these studies.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined comparing the distributional range in the reference period with the present distributional range.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined by comparing its extent when evaluated against the threshold given for the extent of that habitat.
BLK-RO-RG-Coast: Good conservation status for the habitat will be defined by attaining the thresholds set for density / biomass / population structure of the species that are characteristic, key or structural for that particular habitat type.
BLK-RO-RG-Coast: Virtual population analysis for fishing stock assessment (XSA, ICA - SPR, XSA - TUR, VIT4WIND - DGS, VPA XSA - ANE, WHG)
BLK-RO-RG-Marine: Good conservation status for dolphin population will be defined comparing the dolphin stocks with the baseline.
BLK-RO-RG-Coast: Good conservation status for the Cystoseira barbata will be defined comparing the present distributional and population densities range with the baseline.
BLK-RO-RG-Coast: Good conservation status for the Zostera noltii will be defined comparing the present distributional and population densities range with the baseline.
BLK-RO-RG-Coast: For benthic communities (Mytilus galloprovincialis, Modiolula phaseolinus and Lentidium mediterraneum) good environmental status will be defined comparing the present distributional and population densities range with the baseline.

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• Chilia - Periboina
• Periboina-Vama Veche-Mangalia
• marine waters from 1nm to shelf waters (50m isobath), between Sulina - Vama Veche transects

• NutrientLevels
• NutrientOrgEnrich

• MarineCoast
• MarineShelf

• OxygenLevels
• Transparency

5.1

5.1.1

5.2

5.2.1

5.2.2

5.2.3

5.2.4

5.3

5.3.1

5.3.2

5.1.1. For transitional waters, ecological status characterization was performed using "Studies on the development of classification and comprehensive assessment of the status of surface water (transitional waters and coastal waters) as required by the Water Framework Directive 2000/60/EC on the basis of biological chemical and hydromorphological elements", for natural water bodies and heavily modified water bodies", developed by the National Institute for Marine Research and Development,,Grigore Antipa". The period studied was 2007-2011, data from national monitoring network. Determined by comparing annual averages of concentrations in the water column, it was found that N-NH4 values ​​were exceeded in the whole period, O2 values ​​were rated in good ecological condition, and the values ​​N-NO3, N-NO2, P-PO4, BOD5 reached benchmark status.

5.2.4. The reference data analysis for the coastal waters emphasized the diatoms dominance with 54% of total species followed by dinoflagellates, 31%. The others group species represented 15. The ecological groups composition, due to salinity resistance, the freshwate - brackishwater species represents 18% the dominance being of marine and brackish water species, 82%. The quality status assessment, based only the phytoplankton taxonomic composition need special adjustments. However, GES could be defined as a stable ratio between diatoms and dinoflagellates nearby land based sources.

5.3.1. GES could be represented by stable spatial distribution of perennial species (Cystoseira, Zostera).

• Chilia - Periboina = 0,03=N-NO2; 0,1=N-NH4; 1,5=N-NO3; 6=BOD5; 6,2=O2; 0,03=P-PO4
• Periboina-Vama Veche-Mangalia = 0,03=N-NO2; 0,1=N-NH4; 1,5=N-NO3; 6=BOD5; 6,2=O2; 0,03=P-PO4

N-NH4=39%

LimitReferencePoint

Reference values ​​for nutrients were assessed based on analysis of historical data from the period 1960-1965 taking into account the median of these values ​​after outliers were canceled. Reference values ​​for oxygenation conditions were assessed based on historical data analysis in the period 1960-1965 taking into account the limits between which stood 80% of values.

Comparison of annual mean values ​​determined threshold values ​​set using "Studies on the development of classification and comprehensive assessment of the status of surface water (transitional waters and coastal waters) as required by the Water Framework Directive 2000/60/EC on the basis of biological chemical and hydromorphological elements, for natural water bodies and heavily modified water bodies", developed by the National Institute for Marine Research and Development,,Grigore Antipa".

There was not developed a national methodology to assess GES according to the prevision of the Commission Decision 2010/477/EU.

There was not developed a national methodology to assess GES according to the prevision of the Commission Decision 2010/477/EU.

The methodology will be developed in the coming period and the results of its applications will be provided in the second cycle of the MSFD reporting.

The methodology will be developed in the coming period and the results of its applications will be provided in the second cycle of the MSFD reporting.

The methodology will be developed in the coming period and the results of its applications will be provided in the second cycle of the MSFD reporting.